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KMID : 1023420140140010001
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Journal of Dental Hygiene Science
2014 Volume.14 No. 1 p.1 ~ p.6
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The Killing Effect of Candida albicans on Hairless Mouse-2 Mouse Tissues by Non-Thermal Atmospheric Pressure Plasma
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Park Sang-Rye
Kim Gyoo-Cheon
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Abstract
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The purpose of this study was to investigate the killing effect of Candida albicans on hairless mouse-2 (HRM-2) mouse tissues. We tested theeffectiveness of a non-thermal atmospheric pressure plasma in killing C. albicans strains. The viability of C. albicans was determined by countingthe colony forming units (CFU), after non-thermal atmospheric pressure plasma treatment. When non-thermal atmospheric pressure plasma wasrepeatedly treated on mouse skin which inoculated with C. albicans. The C. albicans cells were planted on skin tissue, and then the infected mousetissue was exposed to non-thermal atmospheric pressure plasma for 0 sec, 60 sec, 180 sec and 300 sec. The death rate of C. albicans wasincreased in dependent with treatment times. The three times of non-thermal atmospheric pressure plasma at the interval of 10 minutes significantlyshowed the 6 log CFU/ml reduction of death rate on HRM-2 mouse tissues. Thus, non-thermal atmospheric pressure plasma could be used forthe disinfection of C. albicans on oral surface.
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KEYWORD
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Candida albicans , Colony forming unit , Hariless mouse , Non-thermal atmospheric pressure plasma
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